Pamukkale Ekspresi Istanbul-Denizli

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The Pamukkale Ekspresi night train between Istanbul and Denizli (for Pamukkale) has not been running since 2008 due to track repairs and upgrades.

In July, 2009, the Turkish State Railways revised their list of main-line intercity trains, and the Pamukkale Ekspresi was not in the list.

I don't think it will run again. The Turkish State Railways seems to be moving away from sleeper trains and concentrating on high-speed trains, so I would not encourage you to wait for the return of this very useful overnight train.

Your alternatives are rental car, bus and plane.